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human non small cell lung cancer nsclc cell lines a549  (ATCC)


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    ATCC human non small cell lung cancer nsclc cell lines a549
    Human Non Small Cell Lung Cancer Nsclc Cell Lines A549, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 9086 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+non+small+cell+lung+cancer+cell+line+a549/A549/pm41946943-73-0-15
    Average 99 stars, based on 9086 article reviews
    human non small cell lung cancer nsclc cell lines a549 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Mutagenesis:

    Article Title: N-glycosylation of PD-L1 modulates the efficacy of immune checkpoint blockades targeting PD-L1 and PD-1.
    Article Snippet: IcAR-PD-1 (Mouse BW5147 thymoma, ATCC TIB-47) cells were maintained in RPMI 1640 medium supplemented with 10% (v/v) fetal bovine serum (FBS), penicillin, streptomycin, glutamine, and sodium pyruvate (1 mM each). .. Human breast cancer cell lines MDAMB231(ATCC HTB-26) and MCF7 (ATCC HTC-22), human melanoma cell line A375 (ATCC CRL-1619) and human non-small cell lung cancer cell line A549 (NSCLC; ATCC CCL-185) were transduced to express WT PD-L1 and all PD-L1 mutant variants as well as pQCXIP alone, as a control; the mutants were described in our previous publication ([32]; The current study demonstrates new flow cytometry validations). ..

    Article Title: N-glycosylation of PD-L1 modulates the efficacy of immune checkpoint blockades targeting PD-L1 and PD-1
    Article Snippet: IcAR-PD-1 (Mouse BW5147 thymoma, ATCC TIB-47) cells were maintained in RPMI 1640 medium supplemented with 10% (v/v) fetal bovine serum (FBS), penicillin, streptomycin, glutamine, and sodium pyruvate (1 mM each). .. Human breast cancer cell lines MDA-MB231(ATCC HTB-26) and MCF7 (ATCC HTC-22), human melanoma cell line A375 (ATCC CRL-1619) and human non-small cell lung cancer cell line A549 (NSCLC; ATCC CCL-185) were transduced to express WT PD-L1 and all PD-L1 mutant variants as well as pQCXIP alone, as a control; the mutants were described in our previous publication ([ ]; The current study demonstrates new flow cytometry validations). ..

    Control:

    Article Title: N-glycosylation of PD-L1 modulates the efficacy of immune checkpoint blockades targeting PD-L1 and PD-1.
    Article Snippet: IcAR-PD-1 (Mouse BW5147 thymoma, ATCC TIB-47) cells were maintained in RPMI 1640 medium supplemented with 10% (v/v) fetal bovine serum (FBS), penicillin, streptomycin, glutamine, and sodium pyruvate (1 mM each). .. Human breast cancer cell lines MDAMB231(ATCC HTB-26) and MCF7 (ATCC HTC-22), human melanoma cell line A375 (ATCC CRL-1619) and human non-small cell lung cancer cell line A549 (NSCLC; ATCC CCL-185) were transduced to express WT PD-L1 and all PD-L1 mutant variants as well as pQCXIP alone, as a control; the mutants were described in our previous publication ([32]; The current study demonstrates new flow cytometry validations). ..

    Article Title: N-glycosylation of PD-L1 modulates the efficacy of immune checkpoint blockades targeting PD-L1 and PD-1
    Article Snippet: IcAR-PD-1 (Mouse BW5147 thymoma, ATCC TIB-47) cells were maintained in RPMI 1640 medium supplemented with 10% (v/v) fetal bovine serum (FBS), penicillin, streptomycin, glutamine, and sodium pyruvate (1 mM each). .. Human breast cancer cell lines MDA-MB231(ATCC HTB-26) and MCF7 (ATCC HTC-22), human melanoma cell line A375 (ATCC CRL-1619) and human non-small cell lung cancer cell line A549 (NSCLC; ATCC CCL-185) were transduced to express WT PD-L1 and all PD-L1 mutant variants as well as pQCXIP alone, as a control; the mutants were described in our previous publication ([ ]; The current study demonstrates new flow cytometry validations). ..

    Flow Cytometry:

    Article Title: N-glycosylation of PD-L1 modulates the efficacy of immune checkpoint blockades targeting PD-L1 and PD-1.
    Article Snippet: IcAR-PD-1 (Mouse BW5147 thymoma, ATCC TIB-47) cells were maintained in RPMI 1640 medium supplemented with 10% (v/v) fetal bovine serum (FBS), penicillin, streptomycin, glutamine, and sodium pyruvate (1 mM each). .. Human breast cancer cell lines MDAMB231(ATCC HTB-26) and MCF7 (ATCC HTC-22), human melanoma cell line A375 (ATCC CRL-1619) and human non-small cell lung cancer cell line A549 (NSCLC; ATCC CCL-185) were transduced to express WT PD-L1 and all PD-L1 mutant variants as well as pQCXIP alone, as a control; the mutants were described in our previous publication ([32]; The current study demonstrates new flow cytometry validations). ..

    Article Title: N-glycosylation of PD-L1 modulates the efficacy of immune checkpoint blockades targeting PD-L1 and PD-1
    Article Snippet: IcAR-PD-1 (Mouse BW5147 thymoma, ATCC TIB-47) cells were maintained in RPMI 1640 medium supplemented with 10% (v/v) fetal bovine serum (FBS), penicillin, streptomycin, glutamine, and sodium pyruvate (1 mM each). .. Human breast cancer cell lines MDA-MB231(ATCC HTB-26) and MCF7 (ATCC HTC-22), human melanoma cell line A375 (ATCC CRL-1619) and human non-small cell lung cancer cell line A549 (NSCLC; ATCC CCL-185) were transduced to express WT PD-L1 and all PD-L1 mutant variants as well as pQCXIP alone, as a control; the mutants were described in our previous publication ([ ]; The current study demonstrates new flow cytometry validations). ..

    Cell Culture:

    Article Title: Palmitoylation-dependent regulation of GPX4 suppresses ferroptosis.
    Article Snippet: .. Cell lines and cell culture Human melanoma cell lines A375, SK-MEL-28, and A2058, human colorectal cancer cell lines SW480, SW620, RKO, HT-29, and HCT116, human fibrosarcoma cell line HT1080, human non-small cell lung cancer cell line A549, human breast cancer cell line MCF7, human pancreatic cancer cell line PANC-1, human cervical cancer cell line HeLa, human embryonic kidney cell line 293T, and mouse melanoma cell line B16-F0 were obtained from the American Type Culture Collection (ATCC). ..

    Article Title: Discovery of novel sitolactone derivative leading to PANoptosis and differentiation of acute myeloid leukemia cells.
    Article Snippet: Acute Myeloid Leukemia (AML) is a devastating hematologic malignancy.. Chemotherapy remains the primary treatment, offering rapid disease control and potential complete remission.. However, more than half of the patients develop resistance and relapse, significantly reducing patient survival.

    Multiple Displacement Amplification:

    Article Title: Discovery of novel sitolactone derivative leading to PANoptosis and differentiation of acute myeloid leukemia cells.
    Article Snippet: Acute Myeloid Leukemia (AML) is a devastating hematologic malignancy.. Chemotherapy remains the primary treatment, offering rapid disease control and potential complete remission.. However, more than half of the patients develop resistance and relapse, significantly reducing patient survival.



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    ATCC human non small cell lung cancer cell lines a549
    Viability of tumor cells co-cultured with activated Jurkat cells and incubated with different antibodies. ( A ) Viability of tumor cells co-cultured with Jurkat cells incubated with the indicated antibodies for 48 h. In the <t> and Transwell" and "MGC-803 and Transwell" group, Jurkat cells were added to the upper compartment of the Transwell inserts and A549/MGC-803 were seeded in the lower compartment to avoid direct contact between cell types. The results are expressed as the mean ± SD from triplicates. * P < 0.05, ** P < 0.01. ( B ) Image of A549 cells and MGC-803 cells co-cultured with Jurkat cells after incubation with the indicated antibodies for 48 h by optical microscopy. " width="250" height="auto" />
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    ATCC human non small cell lung cancer a549 cell line
    Viability of tumor cells co-cultured with activated Jurkat cells and incubated with different antibodies. ( A ) Viability of tumor cells co-cultured with Jurkat cells incubated with the indicated antibodies for 48 h. In the <t> and Transwell" and "MGC-803 and Transwell" group, Jurkat cells were added to the upper compartment of the Transwell inserts and A549/MGC-803 were seeded in the lower compartment to avoid direct contact between cell types. The results are expressed as the mean ± SD from triplicates. * P < 0.05, ** P < 0.01. ( B ) Image of A549 cells and MGC-803 cells co-cultured with Jurkat cells after incubation with the indicated antibodies for 48 h by optical microscopy. " width="250" height="auto" />
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    ATCC culture conditions human non small cell lung cancer nsclc cell lines
    Viability of tumor cells co-cultured with activated Jurkat cells and incubated with different antibodies. ( A ) Viability of tumor cells co-cultured with Jurkat cells incubated with the indicated antibodies for 48 h. In the <t> and Transwell" and "MGC-803 and Transwell" group, Jurkat cells were added to the upper compartment of the Transwell inserts and A549/MGC-803 were seeded in the lower compartment to avoid direct contact between cell types. The results are expressed as the mean ± SD from triplicates. * P < 0.05, ** P < 0.01. ( B ) Image of A549 cells and MGC-803 cells co-cultured with Jurkat cells after incubation with the indicated antibodies for 48 h by optical microscopy. " width="250" height="auto" />
    Culture Conditions Human Non Small Cell Lung Cancer Nsclc Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Viability of tumor cells co-cultured with activated Jurkat cells and incubated with different antibodies. ( A ) Viability of tumor cells co-cultured with Jurkat cells incubated with the indicated antibodies for 48 h. In the

    Journal: Scientific Reports

    Article Title: Novel anti-LAG-3 antibody LBL-007 with anti-PD-1 blockade enhances antitumor immunity by promoting T cell-induced apoptosis

    doi: 10.1038/s41598-025-21400-z

    Figure Lengend Snippet: Viability of tumor cells co-cultured with activated Jurkat cells and incubated with different antibodies. ( A ) Viability of tumor cells co-cultured with Jurkat cells incubated with the indicated antibodies for 48 h. In the "A549 and Transwell" and "MGC-803 and Transwell" group, Jurkat cells were added to the upper compartment of the Transwell inserts and A549/MGC-803 were seeded in the lower compartment to avoid direct contact between cell types. The results are expressed as the mean ± SD from triplicates. * P < 0.05, ** P < 0.01. ( B ) Image of A549 cells and MGC-803 cells co-cultured with Jurkat cells after incubation with the indicated antibodies for 48 h by optical microscopy.

    Article Snippet: Human non-small cell lung cancer cell lines A549 and PC-9, human esophagogastric adenocarcinoma cell lines HGC-27 and MGC-803, and the human T-lymphoblastic leukemia Jurkat cell line were obtained from the American Type Culture Collection (ATCC) (Manassas, VA, USA).

    Techniques: Cell Culture, Incubation, Microscopy

    LBL-007 and anti-PD-1 antibodies promote IL-2, IL-10, and TNF production. ( A ) ELISA of IL-2, IL-10, and TNF in the supernatants of the co-culture model after incubation with the indicated antibodies for 48 h. “A549” refers to the Jurkat-A549 co-culture model,

    Journal: Scientific Reports

    Article Title: Novel anti-LAG-3 antibody LBL-007 with anti-PD-1 blockade enhances antitumor immunity by promoting T cell-induced apoptosis

    doi: 10.1038/s41598-025-21400-z

    Figure Lengend Snippet: LBL-007 and anti-PD-1 antibodies promote IL-2, IL-10, and TNF production. ( A ) ELISA of IL-2, IL-10, and TNF in the supernatants of the co-culture model after incubation with the indicated antibodies for 48 h. “A549” refers to the Jurkat-A549 co-culture model, "MGC-803" refers to the Jurkat-MGC-803 co-culture model. ( B ) qPCR of IL-2, IL-10, and TNF in cells from the Jurkat-A549 co-culture model (upper layer) or the Jurkat-MGC-803 co-culture model (lower layer) after incubation with the indicated antibodies for 48 h. The results are expressed as the relative expression of the gene normalized to the endogenous control and to the sample with the lowest expression set as 1 in each type of cell. The results are expressed as the mean ± SD from triplicates. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Article Snippet: Human non-small cell lung cancer cell lines A549 and PC-9, human esophagogastric adenocarcinoma cell lines HGC-27 and MGC-803, and the human T-lymphoblastic leukemia Jurkat cell line were obtained from the American Type Culture Collection (ATCC) (Manassas, VA, USA).

    Techniques: Enzyme-linked Immunosorbent Assay, Co-Culture Assay, Incubation, Expressing, Control